atrosab  (Thermo Fisher)

Journal: Cell Communication and Signaling : CCS

Article Title: Helicobacter pylori infection promotes M1 macrophage polarization and gastric inflammation by activation of NLRP3 inflammasome via TNF/TNFR1 axis

doi: 10.1186/s12964-024-02017-7

Figure Lengend Snippet: TNFα stimulates NLRP3 inflammasome activation in macrophages. A KEGG pathway enrichment analysis was performed using the upregulated genes in NLRP3 high macrophages, in comparison with the NLRP3 low macrophages, according to our previous scRNA data of human gastric tissues. The dotted red box marked the enriched TNF signaling pathway. B Western blots showing the levels of NLRP3 inflammasome-related proteins in THP1 cells treated with different concentrations of TNF-α (0, 100, 200, and 400 ng/ml). C Western blots displaying the expression of NLRP3 inflammasome proteins in THP1 cells treated with TNF-α (200 ng/ml) in combination with TNFα inhibitor QNZ (400 ng/ml). D Western blots presenting the levels of NLRP3 inflammasome-related proteins in THP1 cells treated with TNF-α (200 ng/ml) in combination with TNFR1 antagonist Atrosab (1 μM). E and F qRT-PCR analysis of marker genes for M1 macrophages, including TNF-α , IL-1β , IL-6 , CCL2 , and CCL3 , in THP1 cells, following stimulation with TNF and TNFα inhibitor QNZ ( E ), or with TNF and TNFR1 antagonist Atrosab ( F ). * P < 0.05 , **P < 0.01 , *** P < 0.001

Article Snippet: Atrosab (#HY-P990008), Nigericin (#HY-127019) and LPS (#HY-D1056) were obtained from MedChemExpress (Shanghai, China), and QNZ (#S4902) was purchased from Selleck Chemicals (HOU, USA).

Techniques: Activation Assay, Comparison, Western Blot, Expressing, Quantitative RT-PCR, Marker

Journal: Cell Communication and Signaling : CCS

Article Title: Helicobacter pylori infection promotes M1 macrophage polarization and gastric inflammation by activation of NLRP3 inflammasome via TNF/TNFR1 axis

doi: 10.1186/s12964-024-02017-7

Figure Lengend Snippet: H. pylori induces NLRP3 inflammasome and promotes M1 macrophage polarization through TNFα. A The UMAP plots indicated TNF expression in human gastric tissues from our scRNA-seq data. B Immunofluorescence staining for the co-expression of CD68 (green) and TNFα (red) in human gastritis tissues with or without H. pylori infection. Scale bar, 10 μm. C Western blot assay for TNFα protein expression in THP1 cells infected with H. pylori at various MOIs for 24 h (upper panel), or treated with H. pylori at an MOI of 100 for indicated time points (lower panel). D ELISA assay for detection of the TNF-α concentration in the supernatant of THP1 cells following H. pylori infection with indicated MOI (left panel) or at indicated time points (right panel). E Western blots for the TNFα expression in THP1 cells infected with H. pylori 26,695 strain or its VacA − mutant. F and G Western blots for the protein expression of NLRP3 inflammasome in THP1 cells treated with TNF-α in combination with TNFα inhibitor QNZ ( F ), or in combination with TNFR1 antagonist Atrosab ( G ). H and I The qRT-PCR analysis of mRNA levels of M1 macrophage signature genes in THP1 cells treated with TNF-α in combination with QNZ ( H ), or in combination with Atrosab ( I ). * P < 0.05 , **P < 0.01

Article Snippet: Atrosab (#HY-P990008), Nigericin (#HY-127019) and LPS (#HY-D1056) were obtained from MedChemExpress (Shanghai, China), and QNZ (#S4902) was purchased from Selleck Chemicals (HOU, USA).

Techniques: Expressing, Immunofluorescence, Staining, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay, Mutagenesis, Quantitative RT-PCR

Journal: Journal of Translational Medicine

Article Title: Exploring TNFR1: from discovery to targeted therapy development

doi: 10.1186/s12967-025-06122-0

Figure Lengend Snippet: Clinical trials of anti-TNFR1 agents

Article Snippet: Atrosab , TNFR1 antagonists (Monoclonal antibody) , DRKS00004400 , I # , Inflammatory bowel diseases; Multiple sclerosis; Psoriasis; Rheumatoid arthritis , , Baliopharm AG.

Techniques:

Journal: mAbs

Article Title: ATROSAB, a humanized antagonistic anti-tumor necrosis factor receptor one-specific antibody

doi: 10.4161/mabs.2.6.13583

Figure Lengend Snippet: Table 1

Article Snippet: DNA encoding the light and heavy chain of ATROSAB, including Igκ signal sequences and codon-optimized for production in CHO cells, was produced synthetically (Geneart, Regensburg, Germany).

Techniques: